被招募的巨噬細(xì)胞在炎癥后腹膜微環(huán)境中定殖，會轉(zhuǎn)化為功能上不同的常駐細(xì)胞

中文摘要：

炎癥通常會導(dǎo)致單核細(xì)胞來源的巨噬細(xì)胞募集。這些細(xì)胞的命運(yùn)以及它們在多大程度上能夠獲得常駐巨噬細(xì)胞的身份和功能仍不清楚。在這里，我們顯示，在輕度炎癥期間引入腹腔的巨噬細(xì)胞可以長期存活，但由于持續(xù)存在的常駐巨噬細(xì)胞的存在，它們會保持在未成熟的過渡分化狀態(tài)。相比之下，嚴(yán)重炎癥會導(dǎo)致常駐巨噬細(xì)胞的消失，并出現(xiàn)一個(gè)延長階段，此階段下腹腔無法維持常駐表型，但最終引入的細(xì)胞能夠獲得成熟的常駐身份。這些巨噬細(xì)胞在轉(zhuǎn)錄和功能上也表現(xiàn)出差異，這些差異源于炎癥對腹腔微環(huán)境的改變，并在較小程度上受其來源和駐留時(shí)間的影響。因此，炎癥引發(fā)的腹腔巨噬細(xì)胞的命運(yùn)似乎不是預(yù)先決定的，而是由環(huán)境所調(diào)節(jié)。

英文摘要：

Inflammation generally leads to recruitment of monocyte-derived macrophages. What regulates the fate of these cells and to what extent they can assume the identity and function of resident macrophages is unclear. Here, we show that macrophages elicited into the peritoneal cavity during mild inflammation persist long-term but are retained in an immature transitory state of differentiation due to the presence of enduring resident macrophages. By contrast, severe inflammation results in ablation of resident macrophages and a protracted phase wherein the cavity is incapable of sustaining a resident phenotype, yet ultimately elicited cells acquire a mature resident identity. These macrophages also have transcriptionally and functionally divergent features that result from inflammation-driven alterations to the peritoneal cavity micro-environment and, to a lesser extent, effects of origin and time-of-residency. Hence, rather than being predetermined, the fate of inflammation-elicited peritoneal macrophages seems to be regulated by the environment.

論文信息：

論文題目：Recruited macrophages that colonize the post-inflammatory peritoneal niche convert into functionally divergent resident cells

期刊名稱：Nature Communications

時(shí)間期卷：12, Article number: 1770 (2021)

在線時(shí)間：2021年3月19日

DOI：doi.org/10.1038/s41467-021-21778-0

產(chǎn)品信息：

貨號：CP-005-005

規(guī)格：5ml+5ml

品牌：Liposoma

產(chǎn)地：荷蘭

名稱：Clodronate Liposomes

辦事處：Target Technology(靶點(diǎn)科技)

Clodronate Liposomes氯膦酸鹽脂質(zhì)體清除肝臟和腫瘤巨噬細(xì)胞，疾病模型為：肺癌模型Lewis lung carcinoma (LLC)。荷蘭Liposoma巨噬細(xì)胞清除劑Clodronate Liposomes見刊于Nature Communications：被招募的巨噬細(xì)胞在炎癥后腹膜微環(huán)境中定殖，會轉(zhuǎn)化為功能上不同的常駐細(xì)胞。

Liposoma巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體的材料和方法：

Cell depletion

Sterile peritoneal inflammation

To elicit sterile peritoneal inflammation, mice were injected with 10 or 1000?µg of zymosan A (Sigma-Aldrich) suspended in 200?µl Dulbecco’s PBS (Invitrogen), dPBS or left na?ve as indicated. In some experiments, mice were injected intraperitoneally with 250?µl of 700?nm PKH26-PCL in suspended in Diluent B (Sigma) 24?h prior to zymosan. In some experiments, mice were injected intraperitoneally with 0.0625?mg Clodronate liposomes (Liposoma) suspended in 250?µl dPBS (Gibco). To elicit LPS-induced inflammation mice were injected intraperitoneally with 5?µg of LPS (O111:B4, Sigma-Aldrich) suspended in 200?µl dPBS.

材料和方法文獻(xiàn)截圖：