大腸桿菌自誘導培養基適用于由功能性的lac操縱子(包括lac Y和lac Z)控制的表達系統，如PET系列。表達菌種可用BL21(DE3)。大腸桿菌自誘導培養基(E.coli Auto-Induction Medium)是在LB培養基的基礎上進行改進優化配方，可用于lac調控表達的大腸桿菌菌株培養及蛋白表達。含有多種營養物質并額外添加微量元素、氨基酸等，碳源梯度設計既能滿足菌體生長需求，同時能實現外源蛋白在細菌達到飽和生長期后的自動誘發。同等產量相比傳統LB使用更少培養體積，無需密切監控菌液密度的過程(測OD)，且無需額外添加IPTG。本品同樣適用于多克隆表達，高通量選。產品為粉末形式，500g/瓶，按實驗室已經習慣的常規LB培養基樣配制，配制方便。
LB自誘導培養基LB Broth (Auto Induction Medium)產品介紹選購指南
用于制備不含微量元素的LB培養基的固體粉末，添加有葡萄糖和α-乳糖，以便在大腸桿菌中通過IPTG誘導的啟動子自動誘導蛋白表達。
Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be amber.
Storage: 2oC – 25oC. When not in use, keep container closed to avoid hydration.
Preparation:
Add 34,85g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121oC for 15 to 20 minutes. Store at 2oC to 8oC.
Formulation (g/L)
Tryptone: 10,00
Yeast Extract: 5,00
MgSO4: 0,15
(NH4) 2SO4: 3,30
KH2PO4: 6,80
Na2HPO4: 7,10
Glucose 0,50
Alpha Lactose 2,00
Final pH (25oC): 7,0 ± 0,2
